Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C7M85_RS26310 Genome accession   NZ_CP027766
Coordinates   4871784..4872443 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3342     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4866784..4877443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M85_RS26285 parE 4866875..4868767 (+) 1893 WP_044805641.1 DNA topoisomerase IV subunit B -
  C7M85_RS26290 ygiN 4868815..4869129 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C7M85_RS26295 mdaB 4869160..4869741 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C7M85_RS26300 ygiZ 4870060..4870392 (+) 333 WP_000912120.1 DUF2645 family protein -
  C7M85_RS26305 qseC 4870438..4871787 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C7M85_RS26310 ciaR 4871784..4872443 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C7M85_RS26315 ygiW 4872595..4872987 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C7M85_RS26320 ygiV 4873040..4873522 (+) 483 WP_000183500.1 GyrI-like domain-containing protein -
  C7M85_RS26330 parC 4874068..4876326 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C7M85_RS26335 plsC 4876559..4877296 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=237127 C7M85_RS26310 WP_001221502.1 4871784..4872443(-) (ciaR) [Escherichia coli strain 2013C-3342]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=237127 C7M85_RS26310 WP_001221502.1 4871784..4872443(-) (ciaR) [Escherichia coli strain 2013C-3342]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365