Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CYU58_RS21150 Genome accession   NZ_CP027701
Coordinates   4128816..4129475 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 675SK2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4123816..4134475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYU58_RS21120 (CYU58_21095) ygiS 4125099..4126706 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  CYU58_RS21130 (CYU58_21105) mqsA 4126839..4127234 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  CYU58_RS21135 (CYU58_21110) mqsR 4127236..4127532 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  CYU58_RS21140 (CYU58_21115) ygiV 4127737..4128219 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  CYU58_RS21145 (CYU58_21120) ygiW 4128272..4128664 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CYU58_RS21150 (CYU58_21125) ciaR 4128816..4129475 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  CYU58_RS21155 (CYU58_21130) qseC 4129472..4130821 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  CYU58_RS21160 (CYU58_21135) ygiZ 4130867..4131199 (-) 333 WP_023149630.1 DUF2645 family protein -
  CYU58_RS21170 (CYU58_21145) mdaB 4131518..4132099 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CYU58_RS21175 (CYU58_21150) ygiN 4132130..4132444 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CYU58_RS21180 (CYU58_21155) parE 4132492..4134384 (-) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=237043 CYU58_RS21150 WP_001221493.1 4128816..4129475(+) (ciaR) [Escherichia coli strain 675SK2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=237043 CYU58_RS21150 WP_001221493.1 4128816..4129475(+) (ciaR) [Escherichia coli strain 675SK2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment