Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C7A05_RS04130 Genome accession   NZ_CP027591
Coordinates   577515..578174 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-3011     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 572515..583174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7A05_RS04105 yqiA 572584..573165 (+) 582 WP_000105733.1 esterase YqiA -
  C7A05_RS04110 parE 573194..575085 (+) 1892 Protein_561 DNA topoisomerase IV subunit B -
  C7A05_RS04115 ygiN 575133..575447 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C7A05_RS04120 mdaB 575478..576059 (-) 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
  C7A05_RS04125 qseC 576169..577518 (-) 1350 WP_000673376.1 quorum sensing histidine kinase QseC -
  C7A05_RS04130 ciaR 577515..578174 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C7A05_RS04135 ygiW 578326..578718 (+) 393 WP_001364958.1 OB fold stress tolerance protein YgiW -
  C7A05_RS04140 ygiV 578771..579253 (+) 483 WP_000183486.1 GyrI-like domain-containing protein -
  C7A05_RS04150 parC 579799..582057 (+) 2259 WP_062881426.1 DNA topoisomerase IV subunit A -
  C7A05_RS04155 plsC 582290..583027 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=236452 C7A05_RS04130 WP_001221502.1 577515..578174(-) (ciaR) [Escherichia coli strain 2014C-3011]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=236452 C7A05_RS04130 WP_001221502.1 577515..578174(-) (ciaR) [Escherichia coli strain 2014C-3011]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACTGGCCTTAGTAAAATGGGTTTTAGTGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TTGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment