Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6998_RS17850 Genome accession   NZ_CP027587
Coordinates   3291275..3291934 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-4974     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3286275..3296934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6998_RS17825 plsC 3286422..3287159 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6998_RS17830 parC 3287392..3289650 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6998_RS17840 ygiV 3290196..3290678 (-) 483 WP_000183486.1 GyrI-like domain-containing protein -
  C6998_RS17845 ygiW 3290731..3291123 (-) 393 WP_097453247.1 OB fold stress tolerance protein YgiW -
  C6998_RS17850 ciaR 3291275..3291934 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6998_RS17855 qseC 3291931..3293280 (+) 1350 WP_000673376.1 quorum sensing histidine kinase QseC -
  C6998_RS17860 mdaB 3293390..3293971 (+) 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
  C6998_RS17865 ygiN 3294002..3294316 (+) 315 WP_000958595.1 putative quinol monooxygenase -
  C6998_RS17870 parE 3294364..3296256 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  C6998_RS17875 yqiA 3296285..3296866 (-) 582 WP_000105733.1 esterase YqiA -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=236408 C6998_RS17850 WP_001221502.1 3291275..3291934(+) (ciaR) [Escherichia coli strain 2013C-4974]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=236408 C6998_RS17850 WP_001221502.1 3291275..3291934(+) (ciaR) [Escherichia coli strain 2013C-4974]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACTGGCCTTAGTAAAATGGGTTTTAGTGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TTGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365