Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C7A04_RS08100 Genome accession   NZ_CP027586
Coordinates   1495269..1495928 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2012EL-2448     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1490269..1500928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7A04_RS08075 plsC 1490416..1491153 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C7A04_RS08080 parC 1491386..1493644 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C7A04_RS08090 ygiV 1494190..1494672 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C7A04_RS08095 ygiW 1494725..1495117 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C7A04_RS08100 ciaR 1495269..1495928 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C7A04_RS08105 qseC 1495925..1497274 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C7A04_RS08110 ygiZ 1497320..1497652 (-) 333 WP_000912120.1 DUF2645 family protein -
  C7A04_RS08115 mdaB 1497971..1498552 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C7A04_RS08120 ygiN 1498583..1498897 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C7A04_RS08125 parE 1498945..1500837 (-) 1893 WP_033554192.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=236314 C7A04_RS08100 WP_001221502.1 1495269..1495928(+) (ciaR) [Escherichia coli strain 2012EL-2448]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=236314 C7A04_RS08100 WP_001221502.1 1495269..1495928(+) (ciaR) [Escherichia coli strain 2012EL-2448]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365