Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C7A02_RS16560 Genome accession   NZ_CP027584
Coordinates   3148262..3148921 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 00-3076     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3143262..3153921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7A02_RS16535 plsC 3143409..3144146 (-) 738 WP_000965717.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C7A02_RS16540 parC 3144379..3146637 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C7A02_RS16550 ygiV 3147183..3147665 (-) 483 WP_000183499.1 GyrI-like domain-containing protein -
  C7A02_RS16555 ygiW 3147718..3148110 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C7A02_RS16560 ciaR 3148262..3148921 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C7A02_RS16565 qseC 3148918..3150267 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C7A02_RS16570 ygiZ 3150313..3150645 (-) 333 WP_000912123.1 DUF2645 family protein -
  C7A02_RS16575 mdaB 3150964..3151545 (+) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  C7A02_RS16580 ygiN 3151576..3151890 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C7A02_RS16585 - 3151934..3153265 (-) 1332 WP_000778880.1 MFS transporter -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=236255 C7A02_RS16560 WP_001221502.1 3148262..3148921(+) (ciaR) [Escherichia coli strain 00-3076]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=236255 C7A02_RS16560 WP_001221502.1 3148262..3148921(+) (ciaR) [Escherichia coli strain 00-3076]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment