Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6997_RS29565 Genome accession   NZ_CP027577
Coordinates   5456752..5457411 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-4225     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 5451752..5462411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6997_RS29540 plsC 5451899..5452636 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6997_RS29545 parC 5452869..5455127 (-) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  C6997_RS29555 ygiV 5455673..5456155 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  C6997_RS29560 ygiW 5456208..5456600 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6997_RS29565 ciaR 5456752..5457411 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6997_RS29570 qseC 5457408..5458757 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6997_RS29575 ygiZ 5458803..5459135 (-) 333 WP_000912120.1 DUF2645 family protein -
  C6997_RS29580 mdaB 5459454..5460035 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6997_RS29585 ygiN 5460066..5460380 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C6997_RS29590 parE 5460428..5462320 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=236046 C6997_RS29565 WP_001221502.1 5456752..5457411(+) (ciaR) [Escherichia coli strain 2013C-4225]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=236046 C6997_RS29565 WP_001221502.1 5456752..5457411(+) (ciaR) [Escherichia coli strain 2013C-4225]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365