Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   A2F94_RS21015 Genome accession   NZ_CP027573
Coordinates   3957477..3958136 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-4081     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3952477..3963136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2F94_RS20990 parE 3952568..3954460 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  A2F94_RS20995 ygiN 3954508..3954822 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  A2F94_RS21000 mdaB 3954853..3955434 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  A2F94_RS21005 ygiZ 3955753..3956085 (+) 333 WP_000912120.1 DUF2645 family protein -
  A2F94_RS21010 qseC 3956131..3957480 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  A2F94_RS21015 ciaR 3957477..3958136 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  A2F94_RS21020 ygiW 3958288..3958680 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  A2F94_RS21025 ygiV 3958733..3959215 (+) 483 WP_000183500.1 GyrI-like domain-containing protein -
  A2F94_RS21035 parC 3959761..3962019 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  A2F94_RS21040 plsC 3962252..3962989 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=235943 A2F94_RS21015 WP_001221502.1 3957477..3958136(-) (ciaR) [Escherichia coli strain 2013C-4081]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=235943 A2F94_RS21015 WP_001221502.1 3957477..3958136(-) (ciaR) [Escherichia coli strain 2013C-4081]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365