Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   C6Y56_RS02010 Genome accession   NZ_CP027561
Coordinates   452929..453552 (+) Length   207 a.a.
NCBI ID   WP_169428506.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain G7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447929..458552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6Y56_RS01995 (C6Y56_02015) - 448638..451085 (-) 2448 WP_169428503.1 penicillin-binding protein 1A -
  C6Y56_RS02000 (C6Y56_02020) comM 451302..452366 (+) 1065 WP_169428504.1 pilus assembly protein PilM Machinery gene
  C6Y56_RS02005 (C6Y56_02025) - 452366..452932 (+) 567 WP_169428505.1 PilN domain-containing protein -
  C6Y56_RS02010 (C6Y56_02030) pilO 452929..453552 (+) 624 WP_169428506.1 type 4a pilus biogenesis protein PilO Machinery gene
  C6Y56_RS02015 (C6Y56_02035) comL/pilP 453549..454073 (+) 525 WP_169428507.1 pilus assembly protein PilP Machinery gene
  C6Y56_RS02020 (C6Y56_02040) pilQ 454087..456165 (+) 2079 WP_169428508.1 type IV pilus secretin PilQ Machinery gene
  C6Y56_RS02025 (C6Y56_02045) aroK 456170..456688 (+) 519 WP_011332081.1 shikimate kinase AroK -
  C6Y56_RS02030 (C6Y56_02050) aroB 456831..457931 (+) 1101 WP_169428509.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22968.38 Da        Isoelectric Point: 4.5307

>NTDB_id=235795 C6Y56_RS02010 WP_169428506.1 452929..453552(+) (pilO) [Pseudomonas fluorescens strain G7]
MKPSEWLASLRSIDFNELDTGNIGSWPPAVKGLAGVLLMILVLALGYNFFVSDMESELETKRAEEATLKEQFSSKAHMAA
NLELYTQQMKEMENSFGVLLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQITVTGAYHDLATF
VSGVAGLPRIVTLHDFELAPANPEGGTKLRMSILAKTYRYNDKGLQK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=235795 C6Y56_RS02010 WP_169428506.1 452929..453552(+) (pilO) [Pseudomonas fluorescens strain G7]
ATGAAACCGTCCGAATGGCTGGCCAGCCTGCGCAGTATCGACTTCAACGAGCTGGATACCGGCAACATCGGCTCGTGGCC
ACCGGCAGTAAAAGGCCTGGCCGGAGTGCTGCTGATGATTCTGGTACTGGCGCTGGGCTACAACTTTTTCGTCAGCGACA
TGGAAAGCGAGCTCGAAACCAAACGTGCGGAAGAAGCCACGCTCAAGGAGCAGTTCTCCAGCAAGGCGCACATGGCGGCC
AATCTGGAGCTGTACACCCAGCAGATGAAGGAGATGGAAAACTCGTTCGGCGTGTTGCTGCGGCAATTGCCCAGCGATAC
CGAAGTGCCCGGGTTGCTGGAGGACATCACGCGCACCGGACTGGGAAGTGGCCTTGAGTTCGAAGAGATCAAGCTGTTGC
CGGAAGTAACGCAGCAGTTCTACATCGAATTGCCGATCCAGATCACCGTCACGGGCGCCTATCACGACCTCGCCACATTC
GTCAGCGGCGTGGCCGGATTGCCGCGAATCGTGACCCTGCACGACTTCGAGCTGGCGCCGGCCAACCCGGAAGGCGGGAC
GAAGCTGCGCATGAGCATCCTCGCCAAGACGTACCGCTATAACGACAAGGGGCTGCAAAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

43.878

94.686

0.415
  comO/pilO Acinetobacter baylyi ADP1

44.974

91.304

0.411
  pilO Acinetobacter baumannii D1279779

44.737

91.787

0.411