Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6991_RS21965 Genome accession   NZ_CP027555
Coordinates   3967928..3968587 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3513     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3962928..3973587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6991_RS21940 parE 3963019..3964911 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  C6991_RS21945 ygiN 3964959..3965273 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6991_RS21950 mdaB 3965304..3965885 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6991_RS21955 ygiZ 3966204..3966536 (+) 333 WP_000912120.1 DUF2645 family protein -
  C6991_RS21960 qseC 3966582..3967931 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6991_RS21965 ciaR 3967928..3968587 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6991_RS21970 ygiW 3968739..3969131 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6991_RS21975 ygiV 3969184..3969666 (+) 483 WP_000183500.1 GyrI-like domain-containing protein -
  C6991_RS21985 parC 3970212..3972470 (+) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  C6991_RS21990 plsC 3972703..3973440 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=235762 C6991_RS21965 WP_001221502.1 3967928..3968587(-) (ciaR) [Escherichia coli strain 2013C-3513]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=235762 C6991_RS21965 WP_001221502.1 3967928..3968587(-) (ciaR) [Escherichia coli strain 2013C-3513]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment