Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6953_RS28360 Genome accession   NZ_CP027552
Coordinates   5283027..5283686 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2015C-4498     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 5278027..5288686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6953_RS28335 plsC 5278175..5278912 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6953_RS28340 parC 5279145..5281403 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6953_RS28350 ygiV 5281948..5282430 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  C6953_RS28355 ygiW 5282483..5282875 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6953_RS28360 ciaR 5283027..5283686 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6953_RS28365 qseC 5283683..5285032 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6953_RS28370 ygiZ 5285078..5285410 (-) 333 WP_000912120.1 DUF2645 family protein -
  C6953_RS28375 mdaB 5285729..5286310 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6953_RS28380 ygiN 5286341..5286655 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C6953_RS28385 parE 5286703..5288595 (-) 1893 WP_044805641.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=235703 C6953_RS28360 WP_001221502.1 5283027..5283686(+) (ciaR) [Escherichia coli strain 2015C-4498]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=235703 C6953_RS28360 WP_001221502.1 5283027..5283686(+) (ciaR) [Escherichia coli strain 2015C-4498]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365