Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6950_RS14140 Genome accession   NZ_CP027550
Coordinates   2683459..2684118 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 2015C-4136CT1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2678459..2689118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6950_RS14125 ygiS 2680664..2682271 (-) 1608 WP_001357673.1 ABC transporter substrate-binding protein -
  C6950_RS14130 ygiV 2682380..2682862 (-) 483 WP_000183492.1 GyrI-like domain-containing protein -
  C6950_RS14135 ygiW 2682915..2683307 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6950_RS14140 ciaR 2683459..2684118 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  C6950_RS14145 qseC 2684115..2685464 (+) 1350 WP_001357674.1 quorum sensing histidine kinase QseC -
  C6950_RS14150 mdaB 2685574..2686155 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6950_RS14155 ygiN 2686186..2686500 (+) 315 WP_106878835.1 putative quinol monooxygenase -
  C6950_RS14160 - 2686545..2687432 (-) 888 WP_000614960.1 MurR/RpiR family transcriptional regulator -
  C6950_RS14165 - 2687429..2688376 (-) 948 WP_001296418.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=235579 C6950_RS14140 WP_001221493.1 2683459..2684118(+) (ciaR) [Escherichia coli strain 2015C-4136CT1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=235579 C6950_RS14140 WP_001221493.1 2683459..2684118(+) (ciaR) [Escherichia coli strain 2015C-4136CT1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment