Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6992_RS27595 Genome accession   NZ_CP027548
Coordinates   5159940..5160599 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-3061     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 5154940..5165599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6992_RS27570 parE 5155031..5156923 (+) 1893 WP_000195301.1 DNA topoisomerase IV subunit B -
  C6992_RS27575 ygiN 5156971..5157285 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6992_RS27580 mdaB 5157316..5157897 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6992_RS27585 ygiZ 5158216..5158548 (+) 333 WP_000912120.1 DUF2645 family protein -
  C6992_RS27590 qseC 5158594..5159943 (-) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  C6992_RS27595 ciaR 5159940..5160599 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6992_RS27600 ygiW 5160751..5161143 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6992_RS27605 ygiV 5161196..5161678 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6992_RS27615 parC 5162224..5164482 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6992_RS27620 plsC 5164715..5165452 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=235540 C6992_RS27595 WP_001221502.1 5159940..5160599(-) (ciaR) [Escherichia coli strain 2014C-3061]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=235540 C6992_RS27595 WP_001221502.1 5159940..5160599(-) (ciaR) [Escherichia coli strain 2014C-3061]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365