Detailed information NTG0234976

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: S101392_RS13335
• Genome accession: NZ_CP021921
• Coordinates: 2589680..2590297 (-)
• Length: 205 a.a.
• NCBI ID: WP_088326546.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain SRCM101392
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2584680..2595297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S101392_RS13310 (S101392_02650)	rpsT	2584983..2585249 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
S101392_RS13315 (S101392_02651)	holA	2585264..2586306 (-)	1043	Protein_2583	DNA polymerase III subunit delta	-
S101392_RS21505	-	2586346..2586495 (-)	150	WP_120363468.1	hypothetical protein	-
S101392_RS13320 (S101392_02653)	yqzM	2586536..2586670 (+)	135	WP_003229983.1	YqzM family protein	-
S101392_RS13325 (S101392_02654)	comEC	2586710..2589040 (-)	2331	WP_069322764.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
S101392_RS13330 (S101392_02655)	comEB	2589044..2589613 (-)	570	WP_088326544.1	ComE operon protein 2	-
S101392_RS13335 (S101392_02656)	comEA	2589680..2590297 (-)	618	WP_088326546.1	competence protein ComEA	Machinery gene
S101392_RS13340 (S101392_02657)	comER	2590381..2591202 (+)	822	WP_015714289.1	late competence protein ComER	-
S101392_RS13345	yqeM	2591268..2592010 (-)	743	Protein_2590	class I SAM-dependent methyltransferase	-
S101392_RS13350 (S101392_02660)	rsfS	2592007..2592363 (-)	357	WP_041333759.1	ribosome silencing factor	-
S101392_RS13355 (S101392_02661)	yqeK	2592381..2592941 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
S101392_RS13360 (S101392_02662)	nadD	2592931..2593500 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
S101392_RS13365 (S101392_02663)	yhbY	2593512..2593802 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
S101392_RS13370 (S101392_02664)	aroE	2593796..2594638 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21766.50 Da
• Isoelectric Point: 4.8354

>NTDB_id=234976 S101392_RS13335 WP_088326546.1 2589680..2590297(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM101392]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNMAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=234976 S101392_RS13335 WP_088326546.1 2589680..2590297(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM101392]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCGACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATATGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.049	100	0.98
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376