Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6W78_RS18770 Genome accession   NZ_CP027461
Coordinates   3606049..3606708 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 95-3322     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3601049..3611708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W78_RS18755 ygiS 3603254..3604861 (-) 1608 WP_001468194.1 ABC transporter substrate-binding protein -
  C6W78_RS18760 ygiV 3604970..3605452 (-) 483 WP_000183493.1 GyrI-like domain-containing protein -
  C6W78_RS18765 ygiW 3605505..3605897 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6W78_RS18770 ciaR 3606049..3606708 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  C6W78_RS18775 qseC 3606705..3608054 (+) 1350 WP_000673388.1 quorum sensing histidine kinase QseC -
  C6W78_RS18780 ygiZ 3608100..3608432 (-) 333 WP_042021566.1 DUF2645 family protein -
  C6W78_RS27085 - 3608439..3609149 (-) 711 WP_229467650.1 hypothetical protein -
  C6W78_RS27090 - 3609152..3609631 (-) 480 WP_000065329.1 Hcp family type VI secretion system effector -
  C6W78_RS18790 mdaB 3609881..3610462 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6W78_RS18795 ygiN 3610493..3610807 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=234658 C6W78_RS18770 WP_001221493.1 3606049..3606708(+) (ciaR) [Escherichia coli strain 95-3322]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234658 C6W78_RS18770 WP_001221493.1 3606049..3606708(+) (ciaR) [Escherichia coli strain 95-3322]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment