Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6W76_RS26160 Genome accession   NZ_CP027459
Coordinates   4858120..4858779 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 90-3040     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4853120..4863779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W76_RS26135 ygiN 4854021..4854335 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6W76_RS26140 mdaB 4854366..4854947 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6W76_RS29705 - 4855197..4855676 (+) 480 WP_000065333.1 Hcp family type VI secretion system effector -
  C6W76_RS29710 - 4855679..4856389 (+) 711 WP_032205981.1 hypothetical protein -
  C6W76_RS26150 ygiZ 4856396..4856728 (+) 333 WP_000914691.1 DUF2645 family protein -
  C6W76_RS26155 qseC 4856774..4858123 (-) 1350 WP_032205980.1 quorum sensing histidine kinase QseC -
  C6W76_RS26160 ciaR 4858120..4858779 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6W76_RS26165 ygiW 4858931..4859323 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6W76_RS26170 ygiV 4859376..4859858 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6W76_RS26180 parC 4860404..4862662 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6W76_RS26185 plsC 4862895..4863632 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=234599 C6W76_RS26160 WP_001221502.1 4858120..4858779(-) (ciaR) [Escherichia coli strain 90-3040]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234599 C6W76_RS26160 WP_001221502.1 4858120..4858779(-) (ciaR) [Escherichia coli strain 90-3040]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment