Detailed information NTG0234545

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: S100333_RS13445
• Genome accession: NZ_CP021892
• Coordinates: 2503683..2504300 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain SRCM100333
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2498683..2509300

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S100333_RS13420 (S100333_02743)	rpsT	2498985..2499251 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
S100333_RS13425 (S100333_02744)	holA	2499266..2500309 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
S100333_RS22335	-	2500349..2500498 (-)	150	WP_003229985.1	hypothetical protein	-
S100333_RS13430 (S100333_02745)	yqzM	2500539..2500673 (+)	135	WP_003229983.1	YqzM family protein	-
S100333_RS13435 (S100333_02746)	comEC	2500713..2503043 (-)	2331	WP_069837640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
S100333_RS13440 (S100333_02747)	comEB	2503047..2503616 (-)	570	WP_003229978.1	ComE operon protein 2	-
S100333_RS13445 (S100333_02748)	comEA	2503683..2504300 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
S100333_RS13450 (S100333_02749)	comER	2504384..2505205 (+)	822	WP_032726195.1	late competence protein ComER	-
S100333_RS13455 (S100333_02750)	yqeM	2505271..2506014 (-)	744	WP_069837641.1	class I SAM-dependent methyltransferase	-
S100333_RS13460 (S100333_02751)	rsfS	2506011..2506367 (-)	357	WP_014480315.1	ribosome silencing factor	-
S100333_RS13465 (S100333_02752)	yqeK	2506385..2506945 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
S100333_RS13470 (S100333_02753)	nadD	2506935..2507504 (-)	570	WP_041333756.1	nicotinate-nucleotide adenylyltransferase	-
S100333_RS13475 (S100333_02754)	yhbY	2507516..2507806 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
S100333_RS13480 (S100333_02755)	aroE	2507800..2508642 (-)	843	WP_029317883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=234545 S100333_RS13445 WP_046160608.1 2503683..2504300(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM100333]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=234545 S100333_RS13445 WP_046160608.1 2503683..2504300(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM100333]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376