Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6W74_RS27225 Genome accession   NZ_CP027454
Coordinates   5141259..5141918 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-4423     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 5136259..5146918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W74_RS27200 plsC 5136407..5137144 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6W74_RS27205 parC 5137377..5139635 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6W74_RS27215 ygiV 5140180..5140662 (-) 483 WP_000183502.1 GyrI-like domain-containing protein -
  C6W74_RS27220 ygiW 5140715..5141107 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6W74_RS27225 ciaR 5141259..5141918 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6W74_RS27230 qseC 5141915..5143264 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  C6W74_RS27235 ygiZ 5143310..5143642 (-) 333 WP_000914691.1 DUF2645 family protein -
  C6W74_RS30615 - 5143649..5144359 (-) 711 WP_000834029.1 hypothetical protein -
  C6W74_RS30620 - 5144362..5144841 (-) 480 WP_000065333.1 Hcp family type VI secretion system effector -
  C6W74_RS27245 mdaB 5145091..5145672 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6W74_RS27250 ygiN 5145703..5146017 (+) 315 WP_000958598.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=234450 C6W74_RS27225 WP_001221502.1 5141259..5141918(+) (ciaR) [Escherichia coli strain 2014C-4423]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234450 C6W74_RS27225 WP_001221502.1 5141259..5141918(+) (ciaR) [Escherichia coli strain 2014C-4423]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment