Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6W71_RS14595 Genome accession   NZ_CP027452
Coordinates   2808255..2808914 (-) Length   219 a.a.
NCBI ID   WP_001221478.1    Uniprot ID   B7UIT0
Organism   Escherichia coli strain 2014C-3338     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2803255..2813914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W71_RS14570 - 2803997..2804944 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  C6W71_RS14575 - 2804941..2805828 (+) 888 WP_042103703.1 MurR/RpiR family transcriptional regulator -
  C6W71_RS14580 ygiN 2805873..2806187 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6W71_RS14585 mdaB 2806218..2806799 (-) 582 WP_042103705.1 NADPH:quinone oxidoreductase MdaB -
  C6W71_RS14590 qseC 2806909..2808258 (-) 1350 WP_000673349.1 quorum sensing histidine kinase QseC -
  C6W71_RS14595 ciaR 2808255..2808914 (-) 660 WP_001221478.1 quorum sensing response regulator transcription factor QseB Regulator
  C6W71_RS14600 ygiW 2809066..2809458 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6W71_RS14605 ygiV 2809511..2809993 (+) 483 WP_000183493.1 GyrI-like domain-containing protein -
  C6W71_RS14610 ygiS 2810102..2811709 (+) 1608 WP_000288665.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24701.65 Da        Isoelectric Point: 6.9850

>NTDB_id=234344 C6W71_RS14595 WP_001221478.1 2808255..2808914(-) (ciaR) [Escherichia coli strain 2014C-3338]
MRILLIEDDMLIGDGIKTGLSKMGFSIDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234344 C6W71_RS14595 WP_001221478.1 2808255..2808914(-) (ciaR) [Escherichia coli strain 2014C-3338]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCAT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UIT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365