Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6W68_RS20130 Genome accession   NZ_CP027445
Coordinates   3745923..3746582 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3492     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3740923..3751582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W68_RS20105 ygiN 3741824..3742138 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6W68_RS20110 mdaB 3742169..3742750 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6W68_RS29120 - 3743000..3743479 (+) 480 WP_000065333.1 Hcp family type VI secretion system effector -
  C6W68_RS29125 - 3743482..3744192 (+) 711 WP_032205981.1 hypothetical protein -
  C6W68_RS20120 ygiZ 3744199..3744531 (+) 333 WP_000914691.1 DUF2645 family protein -
  C6W68_RS20125 qseC 3744577..3745926 (-) 1350 WP_032205980.1 quorum sensing histidine kinase QseC -
  C6W68_RS20130 ciaR 3745923..3746582 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6W68_RS20135 ygiW 3746734..3747126 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6W68_RS20140 ygiV 3747179..3747661 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6W68_RS20150 parC 3748207..3750465 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6W68_RS20155 plsC 3750698..3751435 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=234112 C6W68_RS20130 WP_001221502.1 3745923..3746582(-) (ciaR) [Escherichia coli strain 2013C-3492]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234112 C6W68_RS20130 WP_001221502.1 3745923..3746582(-) (ciaR) [Escherichia coli strain 2013C-3492]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365