Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MJ69_RS14870 Genome accession   NZ_CP027442
Coordinates   2630746..2631405 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3252     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2625746..2636405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ69_RS14845 plsC 2625893..2626630 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  MJ69_RS14850 parC 2626863..2629121 (-) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  MJ69_RS14860 ygiV 2629667..2630149 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  MJ69_RS14865 ygiW 2630202..2630594 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MJ69_RS14870 ciaR 2630746..2631405 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  MJ69_RS14875 qseC 2631402..2632751 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  MJ69_RS14880 ygiZ 2632797..2633129 (-) 333 WP_000912120.1 DUF2645 family protein -
  MJ69_RS14885 mdaB 2633448..2634029 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MJ69_RS14890 ygiN 2634060..2634374 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  MJ69_RS14895 parE 2634422..2636314 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=234010 MJ69_RS14870 WP_001221502.1 2630746..2631405(+) (ciaR) [Escherichia coli strain 2013C-3252]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=234010 MJ69_RS14870 WP_001221502.1 2630746..2631405(+) (ciaR) [Escherichia coli strain 2013C-3252]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365