Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6P70_RS24485 Genome accession   NZ_CP027388
Coordinates   4549730..4550389 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2011C-4251     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4544730..4555389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P70_RS24460 parE 4544821..4546713 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  C6P70_RS24465 ygiN 4546761..4547075 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6P70_RS24470 mdaB 4547106..4547687 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6P70_RS24475 ygiZ 4548006..4548338 (+) 333 WP_000912120.1 DUF2645 family protein -
  C6P70_RS24480 qseC 4548384..4549733 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6P70_RS24485 ciaR 4549730..4550389 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6P70_RS24490 ygiW 4550541..4550933 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6P70_RS24495 ygiV 4550986..4551468 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6P70_RS24505 parC 4552014..4554272 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6P70_RS24510 plsC 4554505..4555242 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=233441 C6P70_RS24485 WP_001221502.1 4549730..4550389(-) (ciaR) [Escherichia coli strain 2011C-4251]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=233441 C6P70_RS24485 WP_001221502.1 4549730..4550389(-) (ciaR) [Escherichia coli strain 2011C-4251]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment