Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6P66_RS14550 Genome accession   NZ_CP027373
Coordinates   2799475..2800134 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 05-3629     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2794475..2805134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P66_RS14525 parE 2794566..2796458 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  C6P66_RS14530 ygiN 2796506..2796820 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6P66_RS14535 mdaB 2796851..2797432 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6P66_RS14540 ygiZ 2797751..2798083 (+) 333 WP_000912120.1 DUF2645 family protein -
  C6P66_RS14545 qseC 2798129..2799478 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6P66_RS14550 ciaR 2799475..2800134 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6P66_RS14555 ygiW 2800286..2800678 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6P66_RS14560 ygiV 2800731..2801213 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6P66_RS14570 parC 2801759..2804017 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6P66_RS14575 plsC 2804251..2804988 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=233184 C6P66_RS14550 WP_001221502.1 2799475..2800134(-) (ciaR) [Escherichia coli strain 05-3629]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=233184 C6P66_RS14550 WP_001221502.1 2799475..2800134(-) (ciaR) [Escherichia coli strain 05-3629]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment