Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6P71_RS22595 Genome accession   NZ_CP027368
Coordinates   4324852..4325511 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-3307     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4319852..4330511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P71_RS22570 parE 4319944..4321836 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  C6P71_RS22575 ygiN 4321884..4322198 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C6P71_RS22580 mdaB 4322229..4322810 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6P71_RS22585 ygiZ 4323129..4323461 (+) 333 WP_000912120.1 DUF2645 family protein -
  C6P71_RS22590 qseC 4323507..4324855 (-) 1349 Protein_4141 quorum sensing histidine kinase QseC -
  C6P71_RS22595 ciaR 4324852..4325511 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6P71_RS22600 ygiW 4325663..4326055 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6P71_RS22605 ygiV 4326108..4326590 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6P71_RS22615 parC 4327136..4329394 (+) 2259 WP_106889078.1 DNA topoisomerase IV subunit A -
  C6P71_RS22620 plsC 4329627..4330364 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=233042 C6P71_RS22595 WP_001221502.1 4324852..4325511(-) (ciaR) [Escherichia coli strain 2014C-3307]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=233042 C6P71_RS22595 WP_001221502.1 4324852..4325511(-) (ciaR) [Escherichia coli strain 2014C-3307]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365