Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6P65_RS18470 Genome accession   NZ_CP027366
Coordinates   3558387..3559046 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 89-3156     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3553387..3564046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P65_RS18445 plsC 3553534..3554271 (-) 738 WP_000965717.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6P65_RS18450 parC 3554504..3556762 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6P65_RS18460 ygiV 3557308..3557790 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6P65_RS18465 ygiW 3557843..3558235 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6P65_RS18470 ciaR 3558387..3559046 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6P65_RS18475 qseC 3559043..3560392 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6P65_RS18480 ygiZ 3560438..3560770 (-) 333 WP_000912120.1 DUF2645 family protein -
  C6P65_RS18485 mdaB 3561089..3561670 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6P65_RS18490 ygiN 3561701..3562015 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C6P65_RS18495 parE 3562063..3563955 (-) 1893 WP_106884345.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=232950 C6P65_RS18470 WP_001221502.1 3558387..3559046(+) (ciaR) [Escherichia coli strain 89-3156]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=232950 C6P65_RS18470 WP_001221502.1 3558387..3559046(+) (ciaR) [Escherichia coli strain 89-3156]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment