Detailed information NTG0232914

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CDA09_RS04200
• Genome accession: NZ_CP021731
• Coordinates: 938222..939439 (+)
• Length: 405 a.a.
• NCBI ID: WP_121427469.1
• Uniprot ID: -
• Organism: Azoarcus sp. DN11
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 933222..944439

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDA09_RS04185 (CDA09_04180)	gmhB	935019..935558 (+)	540	WP_121427466.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
CDA09_RS04190 (CDA09_04185)	-	935567..936337 (+)	771	WP_121427467.1	lysophospholipid acyltransferase family protein	-
CDA09_RS04195 (CDA09_04190)	-	936422..938131 (+)	1710	WP_286164374.1	histidine kinase	-
CDA09_RS04200 (CDA09_04195)	pilU	938222..939439 (+)	1218	WP_121427469.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CDA09_RS04205 (CDA09_04200)	-	939663..940229 (-)	567	WP_121427470.1	phasin family protein	-
CDA09_RS04210 (CDA09_04205)	-	940562..941338 (-)	777	WP_121427471.1	enoyl-CoA hydratase	-
CDA09_RS04215 (CDA09_04210)	lptA	941368..941913 (-)	546	WP_121427472.1	lipopolysaccharide transport periplasmic protein LptA	-
CDA09_RS04220 (CDA09_04215)	lptC	941919..942494 (-)	576	WP_121427473.1	LPS export ABC transporter periplasmic protein LptC	-
CDA09_RS04225 (CDA09_04220)	-	942550..943545 (-)	996	WP_121427474.1	KpsF/GutQ family sugar-phosphate isomerase	-

Sequence

Protein

• Length: 405 a.a.
• Molecular weight: 44978.43 Da
• Isoelectric Point: 6.1970

>NTDB_id=232914 CDA09_RS04200 WP_121427469.1 938222..939439(+) (pilU) [Azoarcus sp. DN11]
MSSALGVEEARSYMHRLLNAMHQMEGSDLFISVDFPPSVKAHGTMKALSQQRLTPDITRSLAMSLMNDRQRAEFEAEMEC
NFAISIPGVCRFRVNVFVQQQHAGMVIRTIASEIPTLAKLGLPEVLKEIIMTKRGLVLVVGGTGSGKSTTLAAMIDHRNS
SAPGHIITVEDPVEYVHRNKQCLVTHREVGVDTHSWEHALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLH
ANNANQTIDRIVNFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTISEMIFKGQFQSIKEIMAKS
RELGMRTFDQALFDLYDAGFVSYEEAIRNADSTNELRLQIKLKAERGQPKTSAETELSLSIEEEKEEDAEAEGAAAAPGM
SAAVK

Nucleotide

• Length: 1218 bp

>NTDB_id=232914 CDA09_RS04200 WP_121427469.1 938222..939439(+) (pilU) [Azoarcus sp. DN11]
ATGTCGTCCGCCCTGGGTGTGGAAGAAGCCCGCAGCTACATGCACCGGCTGCTCAACGCGATGCACCAGATGGAGGGATC
CGACCTGTTCATCTCGGTCGACTTCCCGCCCAGCGTGAAGGCGCATGGCACGATGAAGGCGCTCAGCCAGCAGCGCCTGA
CTCCCGACATCACGCGCTCGCTGGCGATGAGCCTGATGAACGATCGCCAGCGCGCCGAGTTCGAGGCCGAGATGGAGTGC
AATTTCGCGATCTCGATCCCGGGCGTGTGCCGCTTCCGCGTGAATGTCTTCGTCCAGCAGCAGCACGCCGGGATGGTCAT
CCGCACCATCGCCTCCGAAATCCCCACGCTGGCCAAGCTCGGCCTGCCCGAGGTGCTCAAGGAGATCATCATGACCAAGC
GCGGCCTCGTGCTGGTCGTGGGCGGCACCGGGTCGGGCAAGTCGACGACGCTGGCGGCGATGATCGACCACCGCAACAGC
TCGGCGCCGGGCCACATCATCACGGTCGAGGATCCGGTCGAATACGTGCATCGTAACAAGCAGTGTCTCGTCACGCATCG
CGAAGTCGGCGTCGACACGCATTCCTGGGAGCACGCGCTGAAAAACACGCTGCGCCAGGCTCCCGACGTGATCCTGATCG
GCGAGATCCGTGACCGCGAGACGATGGAGCACGCGATCGCCTTTGCCGAGACCGGCCACCTGTGTCTTGGCACCCTGCAT
GCGAACAACGCGAACCAGACCATCGACAGGATCGTGAACTTCTTTCCCGAGGAGCGCCGCAACCAGCTGCTGATGGATCT
TTCGTCCAACCTGCGCGCCATCGTCTCGCAGCGCCTGATCCGCACCGAGGACGGCAAGGGGCGCAAAGCGGCCATCGAGA
TCCTGCTCAACACGCCGACGATCTCCGAAATGATCTTCAAGGGACAATTCCAGTCGATCAAGGAGATCATGGCCAAATCG
CGCGAGCTGGGCATGCGCACCTTCGACCAGGCGCTGTTCGATCTCTACGACGCTGGCTTCGTCAGCTACGAGGAGGCCAT
CCGCAATGCCGACTCGACCAACGAGTTGCGCCTGCAGATCAAGCTGAAGGCCGAGCGCGGCCAGCCGAAGACCTCCGCGG
AGACGGAACTGAGCCTGTCGATCGAGGAGGAGAAGGAGGAAGACGCGGAGGCGGAGGGCGCTGCCGCTGCGCCGGGCATG
AGCGCGGCAGTGAAGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (91-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.216	91.358	0.514
pilU	Acinetobacter baylyi ADP1	54.596	88.642	0.484
pilU	Vibrio cholerae strain A1552	51.143	86.42	0.442