Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C6P51_RS13840 Genome accession   NZ_CP027362
Coordinates   2548828..2550036 (-) Length   402 a.a.
NCBI ID   WP_199851718.1    Uniprot ID   -
Organism   Escherichia coli strain 95-3192     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2543828..2555036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P51_RS13820 dam 2544440..2545276 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  C6P51_RS13825 damX 2545383..2546669 (-) 1287 WP_000343195.1 cell division protein DamX -
  C6P51_RS13830 aroB 2546761..2547849 (-) 1089 WP_000439845.1 3-dehydroquinate synthase -
  C6P51_RS13835 aroK 2547906..2548427 (-) 522 WP_000818618.1 shikimate kinase AroK -
  C6P51_RS13840 pilQ 2548828..2550036 (-) 1209 WP_199851718.1 DNA uptake porin HofQ Machinery gene
  C6P51_RS13845 hofP 2549978..2550382 (-) 405 WP_001264141.1 DNA utilization protein HofP -
  C6P51_RS13850 hofO 2550372..2550812 (-) 441 WP_001055740.1 DNA utilization protein HofO -
  C6P51_RS13855 hofN 2550796..2551335 (-) 540 WP_001069342.1 DNA utilization protein HofN -
  C6P51_RS13860 hofM 2551335..2552114 (-) 780 WP_106905648.1 DNA utilization protein HofM -
  C6P51_RS13865 mrcA 2552234..2554786 (+) 2553 WP_001400412.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43566.73 Da        Isoelectric Point: 5.7040

>NTDB_id=232856 C6P51_RS13840 WP_199851718.1 2548828..2550036(-) (pilQ) [Escherichia coli strain 95-3192]
MLIPGVQAVKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILS
VHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDNKTALSALEQW
AAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRINGRLLDLELSA
LEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQV
LQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVS
SE

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=232856 C6P51_RS13840 WP_199851718.1 2548828..2550036(-) (pilQ) [Escherichia coli strain 95-3192]
ATGCTGATACCCGGCGTACAGGCGGTAAAGCCGCAAAAAGTGACGCTGATGGTGGATGACGTTCCGGTAGCTCAGGTGTT
GCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAG
ATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGCCAGGAGGGCAACATTCTCTCA
GTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCT
GGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCTGGGGAAAAGCTACTGAGTGCCAAAG
GGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAACAAAACGGCGTTAAGCGCCCTTGAACAGTGG
GCAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATATTGTCACCATTAATGAAAAAAGTTTGCGTGA
GTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTGGGCAAGTCACCACGCTTGGCAGCGACCTCT
CCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAACGGACGTTTGCTGGATCTTGAGCTTTCTGCG
CTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGCCTCACATCTTCAGCCTGCCAGCATTAAACA
GGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGTCGGTGGAATTTAAAGAGGCCGTCCTGGGGA
TGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTACACATCAGCCAGAACGTTCCGGGGCAGGTG
CTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGAAACGCAGGTCGAGGTCAAAAGCGGAGAAAC
GTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATAGCGTGCCGTTGCTTGGCGACATTCCCTGGT
TCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTAGTGGTGTTTATCACGCCACGACTGGTTTCC
AGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(240-399)

Bacterial type II/III secretion system short domain (Secretin_N)

(110-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.4
  comE Haemophilus influenzae Rd KW20

38.329

100

0.388
  pilQ Vibrio cholerae strain A1552

37.772

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.388
  comE Haemophilus influenzae 86-028NP

39.043

98.756

0.386
  pilQ Pseudomonas aeruginosa PAK

33.945

100

0.368
  comE Glaesserella parasuis strain SC1401

36.453

100

0.368

Multiple sequence alignment