Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6P51_RS12035 Genome accession   NZ_CP027362
Coordinates   2234854..2235513 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 95-3192     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2229854..2240513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6P51_RS12005 ygiS 2231137..2232744 (-) 1608 WP_001400746.1 ABC transporter substrate-binding protein -
  C6P51_RS12015 mqsA 2232877..2233272 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  C6P51_RS12020 mqsR 2233274..2233570 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  C6P51_RS12025 ygiV 2233775..2234257 (-) 483 WP_000183497.1 GyrI-like domain-containing protein -
  C6P51_RS12030 ygiW 2234310..2234702 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6P51_RS12035 ciaR 2234854..2235513 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6P51_RS12040 qseC 2235510..2236858 (+) 1349 Protein_2220 quorum sensing histidine kinase QseC -
  C6P51_RS12045 - 2236940..2237236 (-) 297 WP_000912118.1 DUF2645 family protein -
  C6P51_RS12050 mdaB 2237594..2238175 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6P51_RS12055 ygiN 2238206..2238520 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C6P51_RS12060 parE 2238568..2240460 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=232850 C6P51_RS12035 WP_001221502.1 2234854..2235513(+) (ciaR) [Escherichia coli strain 95-3192]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=232850 C6P51_RS12035 WP_001221502.1 2234854..2235513(+) (ciaR) [Escherichia coli strain 95-3192]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment