Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A3P32_RS04315 Genome accession   NZ_CP021704
Coordinates   899172..900551 (+) Length   459 a.a.
NCBI ID   WP_012845675.1    Uniprot ID   A0A1Z1N7N5
Organism   Lactobacillus johnsonii strain UMNLJ22     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 894172..905551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3P32_RS10595 (A3P32_04455) - 895203..895499 (-) 297 WP_250638270.1 hypothetical protein -
  A3P32_RS10600 (A3P32_04460) - 895550..895762 (-) 213 WP_087713267.1 low temperature requirement protein A -
  A3P32_RS04300 (A3P32_04465) pepC 895922..897271 (-) 1350 WP_087713266.1 aminopeptidase C -
  A3P32_RS10605 - 897469..898020 (+) 552 WP_250638269.1 LCP family protein -
  A3P32_RS10610 - 898050..898502 (+) 453 WP_250638312.1 LCP family protein -
  A3P32_RS04310 (A3P32_04475) - 898621..899172 (+) 552 WP_087713265.1 dUTP diphosphatase -
  A3P32_RS04315 (A3P32_04480) radA 899172..900551 (+) 1380 WP_012845675.1 DNA repair protein RadA Machinery gene
  A3P32_RS04320 (A3P32_04485) gltX 900633..902132 (+) 1500 WP_004895777.1 glutamate--tRNA ligase -
  A3P32_RS04325 (A3P32_04490) cysS 902222..903634 (+) 1413 WP_087713264.1 cysteine--tRNA ligase -
  A3P32_RS04330 (A3P32_04495) - 903627..904070 (+) 444 WP_087713263.1 Mini-ribonuclease 3 -
  A3P32_RS04335 (A3P32_04500) rlmB 904057..904812 (+) 756 WP_004895769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50083.63 Da        Isoelectric Point: 7.2244

>NTDB_id=232660 A3P32_RS04315 WP_012845675.1 899172..900551(+) (radA) [Lactobacillus johnsonii strain UMNLJ22]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSAKATASRLITKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEYSVLYVSGEESASQIKMRADRLGVSDSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQDLKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=232660 A3P32_RS04315 WP_012845675.1 899172..900551(+) (radA) [Lactobacillus johnsonii strain UMNLJ22]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGGAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAGCTAAGGCAACTGCAAGCCGGTTGATAA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGTGCTTTAGCTAAAGAATATAGCGTCTTATATGTTTCAGGAGAAGAGTCTGCGAGTC
AAATAAAGATGCGAGCAGATCGACTAGGAGTAAGCGATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAAAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGACTCTATTCAAACAATGAACGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAATTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGGCATGTAACTAAAGAAGGAGCAATTGCTGGTCCCAAGATTATGGAACATATGGTTGATACCGTCCTTTAC
TTTGAAGGGGATGGGCACCACTCATATCGAATTTTAAGATCAGTCAAGAATCGTTTTGGCGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGATCTTCAGTTGTCGTTTCACTTGAAGGAACGCGGCCGCTTCTTGCGGATATCCAAGCTTTAGTAACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGTATGGCGGTTGCTTCAAGTTACAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTA
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCTGCAAAAGTAGGCTTTAAGAGGATTTTTAT
CCCTAAAAACAACTTAAACCAGGACCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z1N7N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.699

99.782

0.606
  radA Streptococcus pneumoniae Rx1

60.699

99.782

0.606
  radA Streptococcus pneumoniae D39

60.699

99.782

0.606
  radA Streptococcus pneumoniae R6

60.699

99.782

0.606
  radA Streptococcus pneumoniae TIGR4

60.699

99.782

0.606
  radA Streptococcus mitis SK321

60.699

99.782

0.606
  radA Bacillus subtilis subsp. subtilis str. 168

56.579

99.346

0.562

Multiple sequence alignment