Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   ND05_RS27305 Genome accession   NZ_CP027351
Coordinates   5061826..5062485 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2014C-3655     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 5056826..5067485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ND05_RS27280 plsC 5056974..5057711 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  ND05_RS27285 parC 5057944..5060202 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  ND05_RS27295 ygiV 5060747..5061229 (-) 483 WP_000183502.1 GyrI-like domain-containing protein -
  ND05_RS27300 ygiW 5061282..5061674 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  ND05_RS27305 ciaR 5061826..5062485 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  ND05_RS27310 qseC 5062482..5063831 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  ND05_RS27315 ygiZ 5063877..5064209 (-) 333 WP_000914691.1 DUF2645 family protein -
  ND05_RS30735 - 5064216..5064926 (-) 711 WP_000834029.1 hypothetical protein -
  ND05_RS30740 - 5064929..5065408 (-) 480 WP_000065333.1 Hcp family type VI secretion system effector -
  ND05_RS27325 mdaB 5065658..5066239 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  ND05_RS27330 ygiN 5066270..5066584 (+) 315 WP_000958598.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=232655 ND05_RS27305 WP_001221502.1 5061826..5062485(+) (ciaR) [Escherichia coli strain 2014C-3655]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=232655 ND05_RS27305 WP_001221502.1 5061826..5062485(+) (ciaR) [Escherichia coli strain 2014C-3655]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365