Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MJ63_RS16490 Genome accession   NZ_CP027331
Coordinates   3018902..3019561 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3277     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3013902..3024561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ63_RS16465 plsC 3014049..3014786 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  MJ63_RS16470 parC 3015019..3017277 (-) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  MJ63_RS16480 ygiV 3017823..3018305 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  MJ63_RS16485 ygiW 3018358..3018750 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MJ63_RS16490 ciaR 3018902..3019561 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  MJ63_RS16495 qseC 3019558..3020907 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  MJ63_RS16500 ygiZ 3020953..3021285 (-) 333 WP_000912120.1 DUF2645 family protein -
  MJ63_RS16505 mdaB 3021604..3022185 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MJ63_RS16510 ygiN 3022216..3022530 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  MJ63_RS16515 parE 3022578..3024470 (-) 1893 WP_032345138.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=232220 MJ63_RS16490 WP_001221502.1 3018902..3019561(+) (ciaR) [Escherichia coli strain 2013C-3277]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=232220 MJ63_RS16490 WP_001221502.1 3018902..3019561(+) (ciaR) [Escherichia coli strain 2013C-3277]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365