Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   CCX85_RS00680 Genome accession   NZ_CP021640
Coordinates   105000..105434 (+) Length   144 a.a.
NCBI ID   WP_020833199.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain JS12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100000..110434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX85_RS00650 (CCX85_00650) - 101669..102034 (+) 366 WP_002986560.1 DUF1033 family protein -
  CCX85_RS00655 (CCX85_00655) comYA 102127..103065 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  CCX85_RS00660 (CCX85_00660) comYB 103001..104035 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  CCX85_RS00665 (CCX85_00665) comYC 104037..104363 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  CCX85_RS00670 (CCX85_00670) comGD 104338..104766 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  CCX85_RS00675 (CCX85_00675) comGE 104723..105007 (+) 285 WP_265415267.1 competence type IV pilus minor pilin ComGE -
  CCX85_RS00680 (CCX85_00680) comYF 105000..105434 (+) 435 WP_020833199.1 competence type IV pilus minor pilin ComGF Machinery gene
  CCX85_RS00685 (CCX85_00685) comGG 105418..105744 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  CCX85_RS00690 (CCX85_00690) comYH 105842..106795 (+) 954 WP_101248261.1 class I SAM-dependent methyltransferase Machinery gene
  CCX85_RS00695 (CCX85_00695) - 106854..108050 (+) 1197 WP_002987791.1 acetate kinase -
  CCX85_RS00700 (CCX85_00700) - 108237..108545 (+) 309 WP_010921804.1 hypothetical protein -
  CCX85_RS00705 (CCX85_00705) proC 108628..109398 (-) 771 WP_101248262.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16670.28 Da        Isoelectric Point: 10.2526

>NTDB_id=232085 CCX85_RS00680 WP_020833199.1 105000..105434(+) (comYF) [Streptococcus pyogenes strain JS12]
MSKQLSNIKAFTLLDALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVTDNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=232085 CCX85_RS00680 WP_020833199.1 105000..105434(+) (comYF) [Streptococcus pyogenes strain JS12]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGATGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAACTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.36

96.528

0.486
  comYF Streptococcus mutans UA159

49.64

96.528

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

43.284

93.056

0.403
  comGF/cglF Streptococcus pneumoniae Rx1

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae D39

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae R6

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae TIGR4

42.105

92.361

0.389
  comGF/cglF Streptococcus mitis SK321

41.353

92.361

0.382

Multiple sequence alignment