Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6N24_RS17770 Genome accession   NZ_CP027323
Coordinates   3338499..3339158 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3033     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3333499..3344158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6N24_RS17745 plsC 3333646..3334383 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6N24_RS17750 parC 3334616..3336874 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6N24_RS17760 ygiV 3337420..3337902 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C6N24_RS17765 ygiW 3337955..3338347 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6N24_RS17770 ciaR 3338499..3339158 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6N24_RS17775 qseC 3339155..3340504 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6N24_RS17780 ygiZ 3340550..3340882 (-) 333 WP_000912120.1 DUF2645 family protein -
  C6N24_RS17785 mdaB 3341201..3341782 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6N24_RS17790 ygiN 3341813..3342127 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C6N24_RS17795 parE 3342175..3344067 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=232072 C6N24_RS17770 WP_001221502.1 3338499..3339158(+) (ciaR) [Escherichia coli strain 2013C-3033]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=232072 C6N24_RS17770 WP_001221502.1 3338499..3339158(+) (ciaR) [Escherichia coli strain 2013C-3033]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365