Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6N21_RS12535 Genome accession   NZ_CP027317
Coordinates   2347947..2348606 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2015C-3107     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2342947..2353606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6N21_RS12510 plsC 2343095..2343832 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6N21_RS12515 parC 2344065..2346323 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6N21_RS12525 ygiV 2346868..2347350 (-) 483 WP_000183502.1 GyrI-like domain-containing protein -
  C6N21_RS12530 ygiW 2347403..2347795 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6N21_RS12535 ciaR 2347947..2348606 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6N21_RS12540 qseC 2348603..2349952 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  C6N21_RS12545 ygiZ 2349998..2350330 (-) 333 WP_000914691.1 DUF2645 family protein -
  C6N21_RS29990 - 2350337..2351047 (-) 711 WP_000834029.1 hypothetical protein -
  C6N21_RS29995 - 2351050..2351529 (-) 480 WP_000065333.1 Hcp family type VI secretion system effector -
  C6N21_RS12555 mdaB 2351779..2352360 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6N21_RS12560 ygiN 2352391..2352705 (+) 315 WP_000958598.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=231982 C6N21_RS12535 WP_001221502.1 2347947..2348606(+) (ciaR) [Escherichia coli strain 2015C-3107]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=231982 C6N21_RS12535 WP_001221502.1 2347947..2348606(+) (ciaR) [Escherichia coli strain 2015C-3107]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365