Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MJ73_RS22735 Genome accession   NZ_CP027312
Coordinates   4391689..4392348 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2013C-3181     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4386689..4397348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ73_RS22710 parE 4386780..4388672 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  MJ73_RS22715 ygiN 4388720..4389034 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  MJ73_RS22720 mdaB 4389065..4389646 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MJ73_RS22725 ygiZ 4389965..4390297 (+) 333 WP_106918678.1 DUF2645 family protein -
  MJ73_RS22730 qseC 4390343..4391692 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  MJ73_RS22735 ciaR 4391689..4392348 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  MJ73_RS22740 ygiW 4392500..4392892 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MJ73_RS22745 ygiV 4392945..4393427 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  MJ73_RS22755 parC 4393973..4396231 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  MJ73_RS22760 plsC 4396465..4397202 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=231933 MJ73_RS22735 WP_001221502.1 4391689..4392348(-) (ciaR) [Escherichia coli strain 2013C-3181]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=231933 MJ73_RS22735 WP_001221502.1 4391689..4392348(-) (ciaR) [Escherichia coli strain 2013C-3181]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365