Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BJE04_RS14405 Genome accession   NC_005139
Coordinates   3067998..3068588 (-) Length   196 a.a.
NCBI ID   WP_043877335.1    Uniprot ID   -
Organism   Vibrio vulnificus YJ016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3062998..3073588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJE04_RS14385 (VV2988) aroB 3063854..3064945 (-) 1092 WP_011079358.1 3-dehydroquinate synthase -
  BJE04_RS14390 (VV2989) aroK 3064978..3065496 (-) 519 WP_011079357.1 shikimate kinase AroK -
  BJE04_RS14395 (VV2990) pilQ 3065698..3067482 (-) 1785 WP_011151276.1 type IV pilus secretin PilQ Machinery gene
  BJE04_RS14400 (VV2991) pilP 3067496..3068008 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  BJE04_RS14405 (VV2992) pilO 3067998..3068588 (-) 591 WP_043877335.1 type 4a pilus biogenesis protein PilO Machinery gene
  BJE04_RS14410 (VV2993) pilN 3068581..3069171 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  BJE04_RS14415 (VV2994) pilM 3069161..3070168 (-) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  BJE04_RS14420 (VV2995) - 3070308..3072821 (+) 2514 WP_011151280.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22719.22 Da        Isoelectric Point: 5.0836

>NTDB_id=23154 BJE04_RS14405 WP_043877335.1 3067998..3068588(-) (pilO) [Vibrio vulnificus YJ016]
MASWQDLDIDEITDWPILPQAAVVVLLMLIIQGFGYWFYLKPETEVLQRMVAEEQTLKISARIKATKVAALPQLQSQLDE
LTQRYEFLLKQLPVQKELATMLASVNELGIQSKLTFTRIDWGEKQTKDFLYQLPLNIELTGDYHDIGEFSEAIARLPRII
SFTDVTWHRVSMESSTLHFRVKANTYQFKSEAVHEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=23154 BJE04_RS14405 WP_043877335.1 3067998..3068588(-) (pilO) [Vibrio vulnificus YJ016]
ATGGCTAGTTGGCAAGATCTCGATATTGATGAAATCACAGATTGGCCGATTTTACCGCAGGCGGCGGTTGTCGTGTTGCT
GATGTTGATCATTCAAGGTTTTGGTTATTGGTTCTATCTCAAGCCGGAAACGGAAGTGTTGCAAAGGATGGTCGCAGAAG
AGCAAACGCTGAAAATCTCGGCTCGAATTAAGGCGACTAAAGTGGCTGCCTTGCCGCAACTTCAATCGCAACTTGATGAA
CTGACTCAGCGCTATGAATTCTTACTTAAGCAACTGCCCGTGCAGAAGGAGTTGGCGACGATGTTGGCGTCGGTCAATGA
ACTCGGCATTCAAAGTAAGCTCACGTTTACACGTATCGATTGGGGGGAAAAGCAAACCAAGGATTTCCTTTACCAACTGC
CTTTGAATATTGAGTTAACCGGGGATTATCACGATATCGGTGAATTTTCTGAAGCGATTGCCCGCTTGCCTCGCATTATC
AGTTTTACCGATGTGACTTGGCATCGAGTGAGTATGGAAAGCAGCACCTTGCACTTCCGAGTAAAAGCGAACACCTATCA
GTTCAAGTCGGAGGCTGTCCATGAGAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

73.016

96.429

0.704
  pilO Vibrio cholerae strain A1552

67.347

100

0.673

Multiple sequence alignment