Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C6H98_RS23525 Genome accession   NZ_CP027221
Coordinates   4399531..4400190 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 2015C-3101     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4394531..4405190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H98_RS23500 plsC 4394678..4395415 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  C6H98_RS23505 parC 4395648..4397906 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C6H98_RS23515 ygiV 4398452..4398934 (-) 483 WP_000183500.1 GyrI-like domain-containing protein -
  C6H98_RS23520 ygiW 4398987..4399379 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C6H98_RS23525 ciaR 4399531..4400190 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C6H98_RS23530 qseC 4400187..4401536 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C6H98_RS23535 ygiZ 4401582..4401914 (-) 333 WP_000912120.1 DUF2645 family protein -
  C6H98_RS23540 mdaB 4402233..4402814 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C6H98_RS23545 ygiN 4402845..4403159 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C6H98_RS23550 parE 4403207..4405099 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=231446 C6H98_RS23525 WP_001221502.1 4399531..4400190(+) (ciaR) [Escherichia coli strain 2015C-3101]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=231446 C6H98_RS23525 WP_001221502.1 4399531..4400190(+) (ciaR) [Escherichia coli strain 2015C-3101]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365