Detailed information NTG0231409

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: S101267_RS13155
• Genome accession: NZ_CP021505
• Coordinates: 2539930..2540544 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens strain SRCM101267
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2534930..2545544

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S101267_RS13130 (S101267_02635)	rpsT	2535230..2535496 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
S101267_RS13135 (S101267_02636)	holA	2535513..2536554 (-)	1042	Protein_2539	DNA polymerase III subunit delta	-
S101267_RS21450	-	2536594..2536748 (-)	155	Protein_2540	hypothetical protein	-
S101267_RS13140	-	2536789..2536923 (+)	135	WP_003152870.1	YqzM family protein	-
S101267_RS13145 (S101267_02637)	comEC	2536942..2539293 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
S101267_RS13150 (S101267_02638)	-	2539294..2539863 (-)	570	WP_003152868.1	ComE operon protein 2	-
S101267_RS13155 (S101267_02639)	comEA	2539930..2540544 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
S101267_RS13160 (S101267_02640)	comER	2540603..2541424 (+)	822	WP_013352943.1	late competence protein ComER	-
S101267_RS13165 (S101267_02641)	-	2541493..2542230 (-)	738	WP_013352944.1	class I SAM-dependent DNA methyltransferase	-
S101267_RS13170 (S101267_02642)	rsfS	2542227..2542583 (-)	357	WP_003152864.1	ribosome silencing factor	-
S101267_RS13175 (S101267_02643)	yqeK	2542601..2543161 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
S101267_RS13180 (S101267_02644)	-	2543151..2543720 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
S101267_RS13185 (S101267_02645)	yhbY	2543731..2544021 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
S101267_RS13190 (S101267_02646)	aroE	2544015..2544857 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=231409 S101267_RS13155 WP_013352942.1 2539930..2540544(-) (comEA) [Bacillus amyloliquefaciens strain SRCM101267]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=231409 S101267_RS13155 WP_013352942.1 2539930..2540544(-) (comEA) [Bacillus amyloliquefaciens strain SRCM101267]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363