Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   S101359_RS12595 Genome accession   NZ_CP021500
Coordinates   2562583..2563653 (-) Length   356 a.a.
NCBI ID   WP_088117573.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain SRCM101359     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557583..2568653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101359_RS12550 (S101359_02477) tapA 2558118..2558819 (-) 702 WP_088117567.1 amyloid fiber anchoring/assembly protein TapA -
  S101359_RS12555 (S101359_02478) - 2559081..2559404 (+) 324 WP_088117568.1 DUF3889 domain-containing protein -
  S101359_RS12560 (S101359_02479) - 2559451..2559630 (-) 180 WP_003325435.1 YqzE family protein -
  S101359_RS12565 (S101359_02480) comGG 2559700..2560074 (-) 375 WP_088117569.1 competence type IV pilus minor pilin ComGG Machinery gene
  S101359_RS12570 (S101359_02481) comGF 2560075..2560581 (-) 507 WP_274379311.1 competence type IV pilus minor pilin ComGF -
  S101359_RS12575 (S101359_02482) comGE 2560484..2560831 (-) 348 WP_088117570.1 competence type IV pilus minor pilin ComGE Machinery gene
  S101359_RS12580 (S101359_02483) comGD 2560815..2561180 (-) 366 WP_258235513.1 competence type IV pilus minor pilin ComGD Machinery gene
  S101359_RS12585 (S101359_02484) comGC 2561245..2561553 (-) 309 WP_087941811.1 competence type IV pilus major pilin ComGC Machinery gene
  S101359_RS12590 (S101359_02485) comGB 2561559..2562596 (-) 1038 WP_088117572.1 competence type IV pilus assembly protein ComGB Machinery gene
  S101359_RS12595 (S101359_02486) comGA 2562583..2563653 (-) 1071 WP_088117573.1 competence type IV pilus ATPase ComGA Machinery gene
  S101359_RS12605 (S101359_02488) - 2564033..2564986 (-) 954 WP_088117575.1 magnesium transporter CorA family protein -
  S101359_RS12610 (S101359_02489) - 2565098..2566456 (+) 1359 WP_088117576.1 hemolysin family protein -
  S101359_RS12615 (S101359_02490) - 2566523..2567374 (-) 852 WP_088117577.1 STAS domain-containing protein -
  S101359_RS12625 (S101359_02492) - 2567641..2568021 (-) 381 WP_003325422.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  S101359_RS12630 (S101359_02493) - 2568259..2568504 (+) 246 WP_003325421.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40453.77 Da        Isoelectric Point: 9.4624

>NTDB_id=231301 S101359_RS12595 WP_088117573.1 2562583..2563653(-) (comGA) [Bacillus atrophaeus strain SRCM101359]
MDPIEKTSRTLIEEAYSVKASDIHIVPREQDALIHFRIDHALLKKRTMKKEECVRLISHFKFLSAMDIGERRKPQSGSLS
LKLKKGTVYLRMSTLPTINDESLVIRVLPQHNIPAIERLSLFPKTGATLLSFLKHSHGLIIFTGPTGSGKSTTLYSLVQY
AKKHFNRNIITLEDPVETRDQDVLQVQVNEKAGITYSAGLKAILRHDPDMIVLGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTAIAIAAQRLVDLTCPFCENGCSSVYCRITRDVRRATIYELLYGKNLQQCIQEARGE
RANYQYRTLRQIIRKGIALGYLTTNNYDRWVYHEED

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=231301 S101359_RS12595 WP_088117573.1 2562583..2563653(-) (comGA) [Bacillus atrophaeus strain SRCM101359]
TTGGATCCGATAGAAAAAACTAGCAGGACATTAATTGAAGAGGCATATTCCGTGAAGGCCTCGGATATTCACATTGTACC
AAGGGAGCAAGATGCTTTGATCCATTTTCGGATTGACCATGCGTTGCTGAAGAAGCGGACAATGAAAAAGGAAGAGTGCG
TTCGGCTCATTTCCCATTTTAAATTTCTGTCAGCCATGGATATAGGAGAAAGGCGGAAGCCGCAAAGTGGTTCACTTTCT
TTAAAGCTTAAAAAAGGGACAGTTTATCTCAGAATGTCGACACTTCCTACGATTAATGATGAAAGCCTCGTGATCAGAGT
TTTGCCTCAGCATAATATTCCTGCGATCGAAAGACTGTCCCTGTTCCCAAAGACAGGCGCCACGCTGCTATCCTTTTTAA
AGCACTCCCATGGCCTCATTATTTTTACCGGTCCGACTGGCTCAGGGAAATCTACCACCCTATATTCACTGGTTCAATAT
GCAAAAAAGCATTTCAACCGAAATATTATCACTCTCGAAGATCCTGTGGAAACTAGAGATCAGGATGTCTTGCAGGTACA
GGTGAATGAAAAAGCCGGCATTACGTACTCAGCGGGCCTGAAGGCGATTTTGCGCCATGATCCAGACATGATTGTTCTGG
GGGAAATCAGGGATGCTGAAACAGCAAAAATCGCCGTGCGGGCTGCTATGACAGGCCATTTGGTATTAACAAGCCTGCAC
ACAAGAGATGCCAAGGGCGCCATTTACAGACTGCTTGAATTCGGAGTAAATATGAATGAAATAGAGCAAACAGCGATTGC
GATAGCAGCCCAGCGATTAGTCGACTTAACCTGTCCATTTTGTGAAAACGGCTGTTCCTCTGTTTATTGCCGGATTACAC
GTGATGTAAGGCGAGCGACCATTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATACAAGAAGCTAGAGGAGAA
CGGGCCAATTATCAGTACCGAACCTTACGGCAGATTATCAGAAAAGGAATCGCGCTTGGTTATCTAACGACGAATAATTA
CGACCGGTGGGTTTATCATGAAGAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

87.921

100

0.879
  pilB Glaesserella parasuis strain SC1401

44.027

82.303

0.362

Multiple sequence alignment