Detailed information NTG0231225

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: S100757_RS13205
• Genome accession: NZ_CP021499
• Coordinates: 2455756..2456373 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain SRCM100757
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2450756..2461373

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S100757_RS13180 (S100757_02615)	rpsT	2451058..2451324 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
S100757_RS13185 (S100757_02616)	holA	2451339..2452382 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
S100757_RS21245	-	2452422..2452571 (-)	150	WP_003229985.1	hypothetical protein	-
S100757_RS13190 (S100757_02617)	yqzM	2452612..2452746 (+)	135	WP_003229983.1	YqzM family protein	-
S100757_RS13195 (S100757_02618)	comEC	2452786..2455116 (-)	2331	WP_087614626.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
S100757_RS13200 (S100757_02619)	comEB	2455120..2455689 (-)	570	WP_003229978.1	ComE operon protein 2	-
S100757_RS13205 (S100757_02620)	comEA	2455756..2456373 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
S100757_RS13210 (S100757_02621)	comER	2456457..2457278 (+)	822	WP_032726195.1	late competence protein ComER	-
S100757_RS13215 (S100757_02622)	yqeM	2457344..2458087 (-)	744	WP_046160609.1	class I SAM-dependent methyltransferase	-
S100757_RS13220 (S100757_02623)	rsfS	2458084..2458440 (-)	357	WP_014480315.1	ribosome silencing factor	-
S100757_RS13225 (S100757_02624)	yqeK	2458458..2459018 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
S100757_RS13230 (S100757_02625)	nadD	2459008..2459577 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
S100757_RS13235 (S100757_02626)	yhbY	2459589..2459879 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
S100757_RS13240 (S100757_02627)	aroE	2459873..2460715 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=231225 S100757_RS13205 WP_046160608.1 2455756..2456373(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM100757]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=231225 S100757_RS13205 WP_046160608.1 2455756..2456373(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM100757]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376