Detailed information NTG0231146

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: S101444_RS13210
• Genome accession: NZ_CP021498
• Coordinates: 2455733..2456350 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain SRCM101444
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2450733..2461350

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S101444_RS13185 (S101444_02626)	rpsT	2451035..2451301 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
S101444_RS13190 (S101444_02627)	holA	2451316..2452359 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
S101444_RS21245	-	2452399..2452548 (-)	150	WP_003229985.1	hypothetical protein	-
S101444_RS13195 (S101444_02628)	yqzM	2452589..2452723 (+)	135	WP_003229983.1	YqzM family protein	-
S101444_RS13200 (S101444_02629)	comEC	2452763..2455093 (-)	2331	WP_087614626.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
S101444_RS13205 (S101444_02630)	comEB	2455097..2455666 (-)	570	WP_003229978.1	ComE operon protein 2	-
S101444_RS13210 (S101444_02631)	comEA	2455733..2456350 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
S101444_RS13215 (S101444_02632)	comER	2456434..2457255 (+)	822	WP_032726195.1	late competence protein ComER	-
S101444_RS13220 (S101444_02633)	yqeM	2457321..2458064 (-)	744	WP_046160609.1	class I SAM-dependent methyltransferase	-
S101444_RS13225 (S101444_02634)	rsfS	2458061..2458417 (-)	357	WP_014480315.1	ribosome silencing factor	-
S101444_RS13230 (S101444_02635)	yqeK	2458435..2458995 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
S101444_RS13235 (S101444_02636)	nadD	2458985..2459554 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
S101444_RS13240 (S101444_02637)	yhbY	2459566..2459856 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
S101444_RS13245 (S101444_02638)	aroE	2459850..2460692 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=231146 S101444_RS13210 WP_046160608.1 2455733..2456350(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM101444]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=231146 S101444_RS13210 WP_046160608.1 2455733..2456350(-) (comEA) [Bacillus subtilis subsp. subtilis strain SRCM101444]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376