Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GLL_RS13765 Genome accession   NC_005125
Coordinates   2828421..2830052 (-) Length   543 a.a.
NCBI ID   WP_164929076.1    Uniprot ID   -
Organism   Gloeobacter violaceus PCC 7421     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2823421..2835052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLL_RS13740 (glr2661) - 2823578..2824186 (+) 609 WP_011142655.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
  GLL_RS22990 - 2824183..2824386 (+) 204 WP_011142656.1 hypothetical protein -
  GLL_RS13745 (glr2663) - 2824396..2825106 (+) 711 WP_197530003.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
  GLL_RS13750 (gll2664) - 2825263..2825823 (-) 561 WP_011142658.1 Uma2 family endonuclease -
  GLL_RS13755 (gvip375) - 2825985..2827217 (-) 1233 WP_011142659.1 type II secretion system F family protein -
  GLL_RS13760 (gvip376) - 2827238..2828395 (-) 1158 WP_011142660.1 type IV pilus twitching motility protein PilT -
  GLL_RS13765 (gvip377) pilB/pilB1 2828421..2830052 (-) 1632 WP_164929076.1 GspE/PulE family protein Machinery gene
  GLL_RS13770 (glr2668) - 2830221..2830985 (+) 765 WP_011142662.1 sigma-70 family RNA polymerase sigma factor -
  GLL_RS13775 (glr2669) rpaB 2831248..2831973 (+) 726 WP_164929702.1 response regulator transcription factor RpaB -
  GLL_RS13780 (gll2670) - 2832026..2833405 (-) 1380 WP_197530004.1 pyridoxal phosphate-dependent decarboxylase family protein -
  GLL_RS13785 (gll2671) - 2833527..2834189 (-) 663 WP_011142665.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 543 a.a.        Molecular weight: 58472.12 Da        Isoelectric Point: 6.9806

>NTDB_id=23093 GLL_RS13765 WP_164929076.1 2828421..2830052(-) (pilB/pilB1) [Gloeobacter violaceus PCC 7421]
MASAQRLFELKIRYGVAAFDPGGVDPVRLRRLVESWLSIESCAHYRLLPVREEEGVLAVAMVDPERLEALDELQRRGRLR
GLALRRLAIDAQDFATLLAACQAAPMGTDPESAAGELEAPVVRLSSQILDRAVAEGASDIHIEPQPAGVRVRLRRDGVLH
EAGEPLSAELGLALVSRFKILSELDIAERRLPQDGRLHRRVGERAVDFRVSTLPGRFGEKVVLRLLDNAATRLGLDQLIG
EAGTLTAVRELIRRPHGLFLVTGPTGCGKTTTLYAALAERNEPGVNIATAEDPVEYTLAGITQVQVIREKGLDFARILRA
LLRQDPDVVLVGETRDRETAQTAIEAALTGHLVLTSLHTNDAAGAVARLRELGVAPYLIASALVGVLAQRLLRRLCGECK
EAYVPPPERLARFGLTGGTYFRVRGQRGAACPGCGGVGYKGRVGVYEIMSLGEHLRQLIGREATTQTLREAAAGGGMRSL
LAGSLDLARAGVTSLEEVERVTLSDTDQAGSPAAASHPICRGCGGELHSEWPACPYCTLPRID

Nucleotide


Download         Length: 1632 bp        

>NTDB_id=23093 GLL_RS13765 WP_164929076.1 2828421..2830052(-) (pilB/pilB1) [Gloeobacter violaceus PCC 7421]
ATGGCATCCGCCCAGCGATTGTTCGAATTGAAGATCCGCTACGGCGTCGCCGCCTTCGACCCCGGCGGGGTCGATCCGGT
TCGCCTGCGGCGGTTGGTCGAGAGCTGGCTATCTATCGAAAGTTGCGCCCACTACCGGCTGCTGCCCGTGCGCGAGGAGG
AGGGGGTGCTGGCCGTGGCGATGGTCGATCCTGAGCGCCTCGAAGCCCTCGACGAACTGCAGCGGCGGGGGCGGTTGCGC
GGTCTCGCCCTGCGCCGTCTTGCCATCGACGCCCAGGATTTTGCCACGTTGCTTGCAGCCTGCCAGGCAGCGCCGATGGG
GACGGATCCGGAGTCGGCGGCGGGCGAGCTGGAGGCGCCCGTGGTGCGGCTTTCCTCCCAGATTCTCGATCGGGCGGTGG
CGGAAGGGGCCTCCGACATCCACATCGAACCGCAGCCCGCCGGGGTGCGCGTCCGCCTGCGCAGGGACGGTGTCCTGCAC
GAGGCCGGCGAACCGCTGAGCGCCGAGCTGGGATTGGCGCTGGTGTCGCGCTTCAAGATCCTCTCGGAACTGGATATTGC
CGAGCGCCGTCTGCCCCAAGACGGCCGTTTGCACCGCCGCGTCGGGGAGCGGGCCGTCGATTTTCGCGTCTCGACGTTGC
CGGGGCGCTTCGGAGAGAAAGTGGTGCTGAGGCTGCTCGACAACGCCGCCACCCGCTTGGGGCTCGACCAGTTGATTGGC
GAGGCCGGGACGCTCACGGCGGTGCGCGAATTGATTCGTCGCCCCCACGGGCTGTTTCTGGTCACCGGTCCCACCGGCTG
CGGCAAGACGACCACCCTCTACGCGGCGCTTGCCGAGCGCAACGAGCCGGGGGTCAACATCGCCACCGCCGAAGATCCGG
TGGAGTACACCCTGGCGGGGATCACCCAGGTGCAGGTAATCCGCGAGAAGGGCCTCGATTTTGCCCGCATCCTGCGCGCG
CTGCTCCGCCAAGATCCCGACGTCGTGCTGGTGGGGGAGACCCGCGACCGCGAGACCGCCCAGACCGCCATCGAAGCGGC
CCTCACCGGTCACCTGGTGCTCACCAGCCTGCACACCAACGACGCGGCCGGAGCGGTGGCGCGCCTCAGGGAGCTGGGGG
TGGCCCCCTATTTGATTGCCAGTGCCCTGGTGGGCGTGCTCGCCCAGCGGCTGTTGCGCCGGCTTTGCGGCGAATGCAAA
GAGGCGTATGTACCGCCTCCTGAGCGCCTCGCCCGCTTTGGCCTCACAGGCGGGACGTACTTTCGAGTGCGGGGGCAGAG
GGGAGCAGCCTGCCCCGGCTGCGGCGGCGTAGGTTATAAAGGGCGCGTCGGAGTTTACGAAATCATGTCCCTGGGAGAAC
ACCTGCGCCAACTGATCGGCCGCGAAGCCACCACCCAGACGCTGCGCGAGGCGGCGGCGGGGGGCGGGATGCGTTCGCTG
CTGGCAGGTTCTCTAGATCTGGCGCGCGCGGGGGTGACCAGCCTGGAGGAAGTCGAGCGCGTCACCCTGTCCGACACCGA
CCAAGCGGGAAGCCCCGCGGCCGCGAGCCACCCCATCTGCCGCGGCTGCGGCGGCGAGTTGCACAGCGAATGGCCCGCCT
GTCCCTACTGCACTTTGCCCCGGATCGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(4-105)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

49.75

100

0.549
  pilB Vibrio cholerae strain A1552

42.3

89.687

0.379
  pilB Vibrio parahaemolyticus RIMD 2210633

41.498

90.976

0.378
  pilF Thermus thermophilus HB27

42.531

88.766

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.767

87.845

0.376
  pilB Vibrio campbellii strain DS40M4

41.443

89.319

0.37
  pilF Neisseria gonorrhoeae MS11

40.65

90.608

0.368

Multiple sequence alignment