Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CCK83_RS08090 Genome accession   NZ_CP021428
Coordinates   1680700..1681560 (-) Length   286 a.a.
NCBI ID   WP_032038058.1    Uniprot ID   A0A013SYM6
Organism   Acinetobacter pittii strain HUMV-6483     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1675700..1686560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCK83_RS08065 - 1676590..1677900 (+) 1311 WP_087400409.1 tetratricopeptide repeat protein -
  CCK83_RS08070 - 1677977..1678303 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  CCK83_RS08075 rlmB 1678406..1679155 (+) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CCK83_RS08080 - 1679191..1680105 (+) 915 WP_032038060.1 DMT family transporter -
  CCK83_RS08085 coaE 1680102..1680698 (-) 597 WP_032012473.1 dephospho-CoA kinase -
  CCK83_RS08090 pilD 1680700..1681560 (-) 861 WP_032038058.1 prepilin peptidase Machinery gene
  CCK83_RS08095 pilC 1681560..1682786 (-) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  CCK83_RS08100 pilB 1682813..1684525 (-) 1713 WP_032052992.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCK83_RS08105 tpiA 1684817..1685611 (+) 795 WP_017481951.1 triose-phosphate isomerase -
  CCK83_RS08110 secG 1685624..1685953 (+) 330 WP_087400411.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32141.67 Da        Isoelectric Point: 7.1044

>NTDB_id=230676 CCK83_RS08090 WP_032038058.1 1680700..1681560(-) (pilD) [Acinetobacter pittii strain HUMV-6483]
MQEIIAYFIQNLTALYIAVALLSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPELPIIEHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLMLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTLQMLWGLVLTWILIALTFIDFDTQLLPDR
FTLPLAALGLGINTFSIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=230676 CCK83_RS08090 WP_032038058.1 1680700..1681560(-) (pilD) [Acinetobacter pittii strain HUMV-6483]
ATGCAAGAAATTATTGCTTATTTTATTCAAAACTTAACTGCACTTTATATTGCAGTCGCACTATTGAGCCTATGTATTGG
TAGCTTTTTGAATGTGGTAATTTATCGCACCCCCAAAATGATGGAACAAGATTGGCAGCAGGAATGTCAAATGTTGCTTA
ATCCCGAACTACCCATCATTGAGCATGAAAAGCTGACATTAAGTAAACCTGCTTCATCATGTCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCAGTTATAAGCTGGTTAATGTTAAGAGGAAAATGTGGCCATTGCCAACATCCTATTAG
CATTCGTTATCCAGCGATTGAATTACTCACGATGCTATGTTCATTAGTAGTCGTCATGGTTTTTGGTCCAACCCTACAAA
TGCTTTGGGGGCTAGTGCTCACTTGGATTTTGATTGCTTTAACATTTATAGACTTTGATACTCAGCTACTACCTGACCGT
TTCACCCTACCTTTGGCTGCTCTCGGCTTAGGTATTAATACCTTTAGTATTTACACCTCGCCGAACTCAGCCATTTGGGG
ATATTTAATCGGCTTTTTATGTCTTTGGATTGTGTATTACTTATTTAAAGTGATTACAGGCAAAGAAGGTATGGGTTATG
GCGACTTTAAACTGCTTGCTGCCTTAGGTGCATGGATGGGGCCATTGATGCTGCCATTAATTGTGCTATTGTCATCTTTA
CTAGGTGCAATTATTGGCATCATTTTACTAAAATTACGTAATGATAATCAGCCCTTTGCGTTTGGGCCCTATATTGCCAT
TGCGGGTTGGGTTGCCTTTTTATGGGGTGACCAAATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A013SYM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

96.154

100

0.962
  pilD Acinetobacter nosocomialis M2

95.804

100

0.958
  pilD Vibrio cholerae strain A1552

54.212

95.455

0.517
  pilD Vibrio campbellii strain DS40M4

53.309

95.105

0.507
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437

Multiple sequence alignment