Detailed information NTG0230066

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB895_RS11745
• Genome accession: NZ_CP021342
• Coordinates: 2443773..2444891 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain B8342
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2438773..2449891

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB895_RS11710 (AB895_2372)	-	2439602..2440450 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
AB895_RS11715 (AB895_2373)	-	2440703..2441158 (+)	456	WP_029574892.1	bacteriohemerythrin	-
AB895_RS11725 (AB895_2374)	-	2441311..2442378 (+)	1068	WP_004835750.1	hypothetical protein	-
AB895_RS11730 (AB895_2375)	-	2442378..2442701 (+)	324	WP_000442589.1	RnfH family protein	-
AB895_RS11735 (AB895_2376)	-	2442756..2443154 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AB895_RS11740 (AB895_2377)	fur	2443266..2443703 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AB895_RS11745 (AB895_2378)	pilU	2443773..2444891 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB895_RS11750 (AB895_2379)	pilT	2444919..2445956 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AB895_RS11755 (AB895_2380)	-	2446083..2446775 (+)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=230066 AB895_RS11745 WP_000347036.1 2443773..2444891(-) (pilU) [Acinetobacter baumannii strain B8342]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=230066 AB895_RS11745 WP_000347036.1 2443773..2444891(-) (pilU) [Acinetobacter baumannii strain B8342]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392