Detailed information NTG0229909

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CBL09_RS15530
• Genome accession: NZ_CP021326
• Coordinates: 3150415..3151533 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain XH386
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3145415..3156533

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CBL09_RS15520 (CBL09_15385)	-	3148531..3149223 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
CBL09_RS15525 (CBL09_15390)	pilT	3149350..3150387 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
CBL09_RS15530 (CBL09_15395)	pilU	3150415..3151533 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CBL09_RS15535 (CBL09_15400)	fur	3151603..3152040 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
CBL09_RS15540 (CBL09_15405)	-	3152152..3152550 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
CBL09_RS15545 (CBL09_15410)	-	3152605..3152928 (-)	324	WP_000442587.1	RnfH family protein	-
CBL09_RS15550 (CBL09_15415)	-	3152928..3153995 (-)	1068	WP_002019572.1	hypothetical protein	-
CBL09_RS15560 (CBL09_15425)	-	3154148..3154603 (-)	456	WP_000782976.1	bacteriohemerythrin	-
CBL09_RS15565 (CBL09_15430)	-	3154856..3155704 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=229909 CBL09_RS15530 WP_000347039.1 3150415..3151533(+) (pilU) [Acinetobacter baumannii strain XH386]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=229909 CBL09_RS15530 WP_000347039.1 3150415..3151533(+) (pilU) [Acinetobacter baumannii strain XH386]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395