Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ALW85_RS03140 Genome accession   NC_004757
Coordinates   650836..651696 (+) Length   286 a.a.
NCBI ID   WP_011111223.1    Uniprot ID   -
Organism   Nitrosomonas europaea ATCC 19718     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645836..656696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALW85_RS03120 (NE0593) - 646353..647522 (+) 1170 WP_011111219.1 heme biosynthesis protein HemY -
  ALW85_RS03125 (NE0594) - 647527..648573 (-) 1047 WP_011111220.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  ALW85_RS03130 (NE0595) - 648603..649514 (+) 912 WP_011111221.1 SDR family oxidoreductase -
  ALW85_RS03135 (NE0596) pilC 649568..650788 (+) 1221 WP_011111222.1 type II secretion system F family protein Machinery gene
  ALW85_RS03140 (NE0597) pilD 650836..651696 (+) 861 WP_011111223.1 prepilin peptidase Machinery gene
  ALW85_RS03145 (NE0598) coaE 651699..652310 (+) 612 WP_011111224.1 dephospho-CoA kinase -
  ALW85_RS03150 (NE0599) zapD 652384..653139 (+) 756 WP_041357059.1 cell division protein ZapD -
  ALW85_RS13525 - 653156..653350 (+) 195 WP_074671117.1 DNA gyrase inhibitor YacG -
  ALW85_RS03155 (NE0600) lplT 653465..654730 (-) 1266 WP_041356478.1 lysophospholipid transporter LplT -
  ALW85_RS03160 (NE0601) nhaC 654957..656387 (+) 1431 WP_011111227.1 Na+/H+ antiporter NhaC -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31277.09 Da        Isoelectric Point: 7.6852

>NTDB_id=22974 ALW85_RS03140 WP_011111223.1 650836..651696(+) (pilD) [Nitrosomonas europaea ATCC 19718]
MNFLDTLQASSLFFVSFISIIGLLLGSFLNVVIYRLPEMLKREWQQQCAELRGEQIKALPVYNLAIPGSGCPQCGHKISI
LENIPIISYLFLRGQCSGCHTRIPFSYPLVEALTAILSGLAAWHFGFGGLLFAVLVFVWAMIVLTFIDLNTQLLPDSITQ
PLLWTGLIVNLNNGFTDVHSAVVGAVAGYLTLWSVYWLFRLLTGKEGMGYGDFKLLAAIGAWLGWQLLPLVILFSSVVGA
VVGTVLILSAGHSKNMTLPFGPYLAGGGLIALFWGKQINQAYLAMF

Nucleotide


Download         Length: 861 bp        

>NTDB_id=22974 ALW85_RS03140 WP_011111223.1 650836..651696(+) (pilD) [Nitrosomonas europaea ATCC 19718]
ATGAATTTTCTTGATACCCTGCAGGCTTCATCTCTTTTTTTCGTTTCGTTCATCTCGATAATCGGCCTGCTCCTGGGCAG
TTTCCTGAATGTAGTGATTTATCGGCTGCCCGAGATGCTGAAACGTGAGTGGCAGCAACAGTGTGCAGAGCTGCGCGGGG
AACAGATAAAAGCACTGCCGGTATATAACCTGGCTATACCGGGATCCGGTTGTCCGCAGTGTGGGCATAAAATTTCCATT
CTTGAAAATATCCCGATCATCAGTTACCTTTTTTTGCGAGGACAGTGTTCCGGTTGCCATACGCGCATTCCGTTCAGCTA
TCCTCTGGTCGAAGCGCTGACGGCCATTCTGAGCGGTCTGGCCGCTTGGCATTTTGGTTTCGGTGGCCTTCTGTTTGCTG
TCCTGGTTTTTGTGTGGGCGATGATCGTTCTGACCTTTATCGACCTGAATACTCAGCTTTTACCGGACAGTATCACCCAG
CCGTTATTGTGGACAGGGTTGATCGTCAATCTGAACAACGGTTTTACCGATGTTCATTCGGCTGTGGTCGGTGCGGTTGC
AGGTTATCTCACGCTCTGGAGTGTTTACTGGCTGTTCAGATTGCTGACCGGCAAGGAAGGCATGGGATACGGCGATTTCA
AGCTGCTGGCTGCAATCGGTGCCTGGCTGGGCTGGCAATTGCTGCCACTGGTCATTCTGTTTTCTTCTGTGGTGGGTGCC
GTCGTTGGTACTGTTCTGATTCTATCTGCCGGCCATTCGAAAAACATGACACTTCCCTTTGGCCCTTATCTGGCTGGCGG
CGGGCTGATCGCCCTGTTCTGGGGTAAGCAGATCAATCAGGCTTACCTTGCCATGTTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(135-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

50.704

99.301

0.503
  pilD Vibrio cholerae strain A1552

51.648

95.455

0.493
  pilD Neisseria gonorrhoeae MS11

46.691

95.105

0.444
  pilD Acinetobacter baumannii D1279779

45.896

93.706

0.43
  pilD Acinetobacter nosocomialis M2

45.896

93.706

0.43

Multiple sequence alignment