Detailed information NTG0229630

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CA924_RS07360
• Genome accession: NZ_CP021281
• Coordinates: 1598394..1599257 (+)
• Length: 287 a.a.
• NCBI ID: WP_011213806.1
• Uniprot ID: I7HZ94
• Organism: Legionella pneumophila subsp. pneumophila strain Flint
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1593394..1604257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CA924_RS07340	-	1593888..1594214 (-)	327	WP_011213802.1	DUF1820 family protein	-
CA924_RS07345	-	1594397..1595140 (-)	744	WP_011213803.1	methyltransferase domain-containing protein	-
CA924_RS07350	pilB	1595399..1597122 (+)	1724	Protein_1415	type IV-A pilus assembly ATPase PilB	-
CA924_RS07355	pilC	1597128..1598348 (+)	1221	WP_011213805.1	type II secretion system F family protein	Machinery gene
CA924_RS07360	pilD	1598394..1599257 (+)	864	WP_011213806.1	A24 family peptidase	Machinery gene
CA924_RS07365	-	1599271..1600476 (-)	1206	WP_011213807.1	cyclic nucleotide-binding domain-containing protein	-
CA924_RS07370	-	1600584..1601177 (-)	594	WP_011213808.1	hypothetical protein	-
CA924_RS07375	-	1601193..1601648 (-)	456	WP_011213809.1	hypothetical protein	-
CA924_RS07380	-	1601782..1602597 (+)	816	WP_011213810.1	pyruvate, water dikinase regulatory protein	-
CA924_RS07385	-	1602717..1604165 (-)	1449	WP_011213811.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32817.40 Da
• Isoelectric Point: 7.8442

>NTDB_id=229630 CA924_RS07360 WP_011213806.1 1598394..1599257(+) (pilD) [Legionella pneumophila subsp. pneumophila strain Flint]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYIFLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=229630 CA924_RS07360 WP_011213806.1 1598394..1599257(+) (pilD) [Legionella pneumophila subsp. pneumophila strain Flint]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCTGTTGGAAGTTT
GCTTAATGTGATAATCTACCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTTTTAAGAGGACGCTGTTACCAATGCGATAGTCCAATTTCAATTCGCTA
CCCTTTTGTTGAGACTCTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAACTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATATATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCTTTATGGCTATTCATTAATCTGTTTTATCTGATGACAGGTAAGGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATTATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: I7HZ94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	48.352	95.122	0.46
pilD	Acinetobacter baumannii D1279779	47.636	95.819	0.456
pilD	Vibrio campbellii strain DS40M4	45.324	96.864	0.439
pilD	Neisseria gonorrhoeae MS11	42.446	96.864	0.411