Detailed information NTG0229131

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CA935_RS07430
• Genome accession: NZ_CP021258
• Coordinates: 1656804..1657667 (+)
• Length: 287 a.a.
• NCBI ID: WP_027227023.1
• Uniprot ID: A0A2S6EZM3
• Organism: Legionella pneumophila subsp. fraseri strain D-5744
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1651804..1662667

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CA935_RS07410	-	1652243..1652569 (-)	327	WP_027227020.1	DUF1820 family protein	-
CA935_RS07415	-	1652752..1653495 (-)	744	WP_027227021.1	methyltransferase domain-containing protein	-
CA935_RS07420	pilB	1653808..1655532 (+)	1725	WP_027227022.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CA935_RS07425	pilC	1655538..1656758 (+)	1221	WP_027221710.1	type II secretion system F family protein	Machinery gene
CA935_RS07430	pilD	1656804..1657667 (+)	864	WP_027227023.1	A24 family peptidase	Machinery gene
CA935_RS07435	-	1657681..1658886 (-)	1206	WP_027227024.1	cyclic nucleotide-binding domain-containing protein	-
CA935_RS07440	-	1658996..1659589 (-)	594	WP_027227025.1	hypothetical protein	-
CA935_RS07445	-	1659605..1660060 (-)	456	WP_027227026.1	hypothetical protein	-
CA935_RS07450	-	1660208..1661023 (+)	816	WP_027227027.1	pyruvate, water dikinase regulatory protein	-
CA935_RS07455	-	1661105..1662559 (-)	1455	WP_027227028.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32867.63 Da
• Isoelectric Point: 8.2759

>NTDB_id=229131 CA935_RS07430 WP_027227023.1 1656804..1657667(+) (pilD) [Legionella pneumophila subsp. fraseri strain D-5744]
MINTLIINYTWFMYLVIGLFSLAVGSLLNVIIYRLPIILQEEWREQCCELLNLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCKSPISIRYPLIELLTTVLSLYASWHFGFTVQLLFVLLAIWILIPLTFIDLDHQLLPDSLTL
GLLWLGLIANTWSIFVSLDIAVLSCAGAYLALWLFINLFYLITGKVGMGHGDFKLFAAFGAWFGWMYLPLILLMSSITGA
IIGLIYLRINGKSRDTAIPFGPFLCISGLIAMFLGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=229131 CA935_RS07430 WP_027227023.1 1656804..1657667(+) (pilD) [Legionella pneumophila subsp. fraseri strain D-5744]
ATGATAAATACGCTAATAATCAATTATACCTGGTTTATGTATTTAGTCATCGGACTCTTTTCTTTGGCCGTTGGAAGTTT
ACTTAATGTTATCATCTATCGCTTACCAATTATTTTGCAGGAAGAATGGCGAGAACAATGCTGTGAGTTACTTAATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATCTATTTTTACCACGTTCATTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCCCTATTAAGCTATATCTTGTTAAGAGGTCGCTGTTACCAATGCAAAAGTCCCATTTCAATTCGCTA
TCCCCTTATTGAGTTATTGACCACAGTGTTATCATTATACGCCAGTTGGCACTTTGGATTTACAGTACAATTATTATTTG
TTTTATTAGCCATCTGGATATTGATACCTCTTACTTTTATTGATCTTGATCATCAATTACTCCCTGATAGTCTCACTTTA
GGACTATTATGGCTTGGTCTTATTGCAAATACATGGAGTATATTTGTTAGTCTTGATATTGCTGTGTTAAGTTGTGCCGG
TGCCTACTTAGCCTTATGGTTATTCATTAATCTATTTTATCTGATTACAGGTAAGGTAGGCATGGGGCATGGTGATTTCA
AATTATTTGCAGCATTTGGCGCATGGTTTGGCTGGATGTATTTACCATTAATTCTTCTGATGTCTTCAATAACCGGAGCT
ATCATTGGTTTGATTTATTTAAGGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGTCCTTTTCTTTGCATTTC
CGGGTTAATAGCTATGTTTTTGGGGGACTCCATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: A0A2S6EZM3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.534	97.909	0.495
pilD	Acinetobacter nosocomialis M2	49.084	95.122	0.467
pilD	Acinetobacter baumannii D1279779	48.364	95.819	0.463
pilD	Vibrio campbellii strain DS40M4	46.403	96.864	0.449
pilD	Neisseria gonorrhoeae MS11	46.043	96.864	0.446