Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C5F74_RS03735 Genome accession   NZ_CP026939
Coordinates   768959..769618 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain CFS3313     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 763959..774618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C5F74_RS03710 (C5F74_04020) - 764615..765946 (+) 1332 WP_000778880.1 MFS transporter -
  C5F74_RS03715 (C5F74_04025) ygiN 765990..766304 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  C5F74_RS03720 (C5F74_04030) mdaB 766335..766916 (-) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  C5F74_RS03725 (C5F74_04035) ygiZ 767235..767567 (+) 333 WP_000912123.1 DUF2645 family protein -
  C5F74_RS03730 (C5F74_04040) qseC 767613..768962 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  C5F74_RS03735 (C5F74_04045) ciaR 768959..769618 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  C5F74_RS03740 (C5F74_04050) ygiW 769770..770162 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C5F74_RS03745 (C5F74_04055) ygiV 770215..770697 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  C5F74_RS03750 (C5F74_04065) parC 771243..773501 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  C5F74_RS03755 (C5F74_04070) plsC 773734..774471 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=229066 C5F74_RS03735 WP_001221502.1 768959..769618(-) (ciaR) [Escherichia coli strain CFS3313]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=229066 C5F74_RS03735 WP_001221502.1 768959..769618(-) (ciaR) [Escherichia coli strain CFS3313]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment