Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B9N48_RS12860 Genome accession   NZ_CP021169
Coordinates   2465078..2465695 (-) Length   205 a.a.
NCBI ID   WP_041850029.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2460078..2470695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9N48_RS12835 rpsT 2460380..2460646 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  B9N48_RS12840 holA 2460661..2461704 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  B9N48_RS20640 - 2461744..2461893 (-) 150 WP_120363468.1 hypothetical protein -
  B9N48_RS12845 yqzM 2461934..2462068 (+) 135 WP_003229983.1 YqzM family protein -
  B9N48_RS12850 comEC 2462108..2464438 (-) 2331 WP_041850028.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B9N48_RS12855 comEB 2464442..2465011 (-) 570 WP_003229978.1 ComE operon protein 2 -
  B9N48_RS12860 comEA 2465078..2465695 (-) 618 WP_041850029.1 competence protein ComEA Machinery gene
  B9N48_RS12865 comER 2465779..2466600 (+) 822 WP_041850030.1 late competence protein ComER -
  B9N48_RS12870 yqeM 2466666..2467409 (-) 744 WP_042976537.1 class I SAM-dependent methyltransferase -
  B9N48_RS12875 rsfS 2467406..2467762 (-) 357 WP_014480315.1 ribosome silencing factor -
  B9N48_RS12880 yqeK 2467780..2468340 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B9N48_RS12885 nadD 2468330..2468899 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  B9N48_RS12890 yhbY 2468911..2469201 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  B9N48_RS12895 aroE 2469195..2470037 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21821.62 Da        Isoelectric Point: 4.7220

>NTDB_id=228782 B9N48_RS12860 WP_041850029.1 2465078..2465695(-) (comEA) [Bacillus subtilis strain TLO3]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=228782 B9N48_RS12860 WP_041850029.1 2465078..2465695(-) (comEA) [Bacillus subtilis strain TLO3]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-117)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38

Multiple sequence alignment